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    Ok, Bilal, thanks for the update.<br>
    It's hard for me to check it now, because I'd have to
    download/compile the entire VV project plus ITK, VTK, etc.<br>
    In any case, I still suggest you to play with the parameters of
    clitkExtracLung related to the extraction of the trachea. You can
    also post the output of --verboseRG on the list.<br>
    Best,<br>
    Rômulo<br>
    <br>
    <div class="moz-cite-prefix">On 23/10/2013 19:20, Bilal Tahir wrote:<br>
    </div>
    <blockquote
cite="mid:CAFZ9P6G_dEiTWKqQvJiZ_8T=2ue--Hi_VK6-TxWHLy=qOCWkhQ@mail.gmail.com"
      type="cite">
      <div dir="ltr">Hi <span
          style="font-family:arial,sans-serif;font-size:12.727272033691406px">Rômulo,</span>
        <div><font face="arial, sans-serif"><br>
          </font></div>
        <div><font face="arial, sans-serif">Thanks for your reply.
            Actually, Vivien had already converted those images from
            matlab format to mhd. I double checked them and he did
            indeed account for the signed/unsigned HU related issues.
            Let me know and I could put one of the images up on dropbox.
            I'm looking at their breath-hold copd data - not 4dct.</font></div>
        <div><font face="arial, sans-serif"><br>
          </font></div>
        <div><font face="arial, sans-serif">Best wishes,</font></div>
        <div><font face="arial, sans-serif"><br>
          </font></div>
        <div><font face="arial, sans-serif">Bilal<br>
          </font>
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      <div class="gmail_extra"><br>
        <br>
        <div class="gmail_quote">On 23 October 2013 21:41, Rômulo Pinho
          <span dir="ltr"><<a moz-do-not-send="true"
              href="mailto:romulopinho@yahoo.com.br" target="_blank">romulopinho@yahoo.com.br</a>></span>
          wrote:<br>
          <blockquote class="gmail_quote" style="margin:0 0 0
            .8ex;border-left:1px #ccc solid;padding-left:1ex">
            <div bgcolor="#FFFFFF" text="#000000"> Hello, Bilal,<br>
              <br>
              I'd have to verify this (maybe Vivien can also comment on
              that), but I think the dir-lab data are not in HU, i.e.,
              the grey values are all positive, from 0 to 4096. The
              algorithms in clitkExtractLung apply some thresholding
              operations that depend on a data set in HU, roughly from
              -1024 to 3072. <br>
              <br>
              To cope with it, you can either play with the parameters
              seedPreProcessingThreshold and upperThresholdForTrachea in
              clitkExtratLung (depending on TYPE)<br>
              "upperThresholdForTrachea"    -    "0: Initial upper
              threshold for trachea"  default="-900"<br>
              "seedPreProcessingThreshold"   -  "1: Threshold for the
              pre-processing step of the algorithm" default="-400"<br>
              <br>
              or, to be on the safe side, shift your grey values prior
              to launching clitkExtractLung. You can do it by creating
              an image with -1024 everywhere (clitkCreateImage does the
              job) and then applying the shift by adding the two images
              with clitkImageArithm. <br>
              <br>
              In any case, using --verboseRG might also give some extra
              insights.<br>
              <br>
              I hope this helps!<br>
              <br>
              Best,<br>
              Rômulo
              <div>
                <div class="h5"><br>
                  <br>
                  <div>On 23/10/2013 14:15, Bilal Tahir wrote:<br>
                  </div>
                  <blockquote type="cite">
                    <div dir="ltr">Thanks Vivien,
                      <div><br>
                      </div>
                      <div>I've got passed the .afdb stage. However I'm
                        getting errors at the 'search for trachea' stage
                        for both the original and LOLA11 algorithms. The
                        dataset is the inspiration scans for patient 1
                        of the dir-lab copd data. </div>
                      <div><br>
                      </div>
                      <div><span style="background-color:rgb(255,0,0)">1)
                          LOLA 11:</span></div>
                      <div><span style="background-color:rgb(255,0,0)"><br>
                        </span></div>
                      <div>
                        <div><span style="background-color:rgb(255,0,0)">[md1bat@testnode02

                            mhd]$ clitkExtractLung --verboseStep 1 -i
                            copd1_00.mhd -a default.afdb -o 00_lungs.mhd
                            --type=1</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Step
                            1 -- Copy and crop input image to 'patient'
                            extends</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Step
                            2 -- Set background to initial image</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Step
                            3 -- Remove Air</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Step
                            4 -- Search for the trachea</span></div>
                        <div><span style="background-color:rgb(255,0,0)"><br>
                          </span></div>
                        <div><span style="background-color:rgb(255,0,0)">itk::InvalidRequestedRegionError

                            (0x2bca660)</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Location:

                            "unknown" </span></div>
                        <div><span style="background-color:rgb(255,0,0)">File:

/home/md1bat/Source/itk/Modules/Core/Common/src/itkDataObject.cxx</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Line:
                            411</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Description:

                            Requested region is (at least partially)
                            outside the largest possible region.</span></div>
                      </div>
                      <div><span style="background-color:rgb(255,0,0)"><br>
                        </span></div>
                      <div><span style="background-color:rgb(255,0,0)">2)
                          Original (type=0)</span></div>
                      <div><span style="background-color:rgb(255,0,0)"><br>
                        </span></div>
                      <div>
                        <div><span style="background-color:rgb(255,0,0)">[md1bat@testnode02

                            mhd]$ clitkExtractLung --verboseStep 1 -i
                            copd1_00.mhd -a default.afdb -o 00_lungs.mhd
                            --type=0</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Step
                            1 -- Copy and crop input image to 'patient'
                            extends</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Step
                            2 -- Set background to initial image</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Step
                            3 -- Remove Air</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Step
                            4 -- Search for the trachea</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4035.53
                            cc) seems not correct. I skip some slices
                            (0) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4037.01
                            cc) seems not correct. I skip some slices
                            (5) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4036.86
                            cc) seems not correct. I skip some slices
                            (10) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4036.39
                            cc) seems not correct. I skip some slices
                            (15) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4036.07
                            cc) seems not correct. I skip some slices
                            (20) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4035.31
                            cc) seems not correct. I skip some slices
                            (25) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4035.54
                            cc) seems not correct. I skip some slices
                            (30) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4035.49
                            cc) seems not correct. I skip some slices
                            (35) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4035.39
                            cc) seems not correct. I skip some slices
                            (40) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4035.57
                            cc) seems not correct. I skip some slices
                            (45) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4035.69
                            cc) seems not correct. I skip some slices
                            (50) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4036.09
                            cc) seems not correct. I skip some slices
                            (55) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)"> 
                                   The volume of the trachea (4036.34
                            cc) seems not correct. I skip some slices
                            (60) and restart to find seeds.</span></div>
                        <div><span style="background-color:rgb(255,0,0)">2
                            : Number of slices to skip to find trachea
                            too high = 65</span></div>
                        <div><span style="background-color:rgb(255,0,0)"><br>
                          </span></div>
                        <div><span style="background-color:rgb(255,0,0)">itk::ExceptionObject

                            (0x377f970)</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Location:

                            "Unknown" </span></div>
                        <div><span style="background-color:rgb(255,0,0)">File:

/home/md1bat/Source/vv/segmentation/clitkExtractLungFilter.txx</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Line:
                            266</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Description:

                            No seeds for trachea... Aborting.</span></div>
                      </div>
                      <div><span style="background-color:rgb(255,0,0)"><br>
                        </span></div>
                      <div><span style="background-color:rgb(255,0,0)">3)
                          Manually setting a higher number of slices
                          than 65</span></div>
                      <div><span style="background-color:rgb(255,0,0)"><br>
                        </span></div>
                      <div>
                        <div><span style="background-color:rgb(255,0,0)">[md1bat@testnode02

                            mhd]$ clitkExtractLung --verbose 1 -i
                            copd1_00.mhd -a default.afdb -o 00_lungs.mhd
                            --skipslices=90</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Input
                            is 3D short.</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Reading

                            [copd1_00.mhd] ... </span></div>
                        <div><span style="background-color:rgb(255,0,0)">2
                            : Number of slices to skip to find trachea
                            too high = 95</span></div>
                        <div><span style="background-color:rgb(255,0,0)"><br>
                          </span></div>
                        <div><span style="background-color:rgb(255,0,0)">itk::ExceptionObject

                            (0x3a379d0)</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Location:

                            "Unknown" </span></div>
                        <div><span style="background-color:rgb(255,0,0)">File:

/home/md1bat/Source/vv/segmentation/clitkExtractLungFilter.txx</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Line:
                            266</span></div>
                        <div><span style="background-color:rgb(255,0,0)">Description:

                            No seeds for trachea... Aborting.</span></div>
                      </div>
                      <div><br>
                      </div>
                      <div>As you can see, I keep failing at the search
                        trachea stage even by trying various different
                        options for slices to skip for the trachea.
                        Please let me know if you have any suggestions
                        that can help.</div>
                      <div><br>
                      </div>
                      <div>Best wishes,</div>
                      <div><br>
                      </div>
                      <div>Bilal</div>
                      <div class="gmail_extra"><br>
                        <br>
                        <div class="gmail_quote">On 23 October 2013
                          16:51, Vivien Delmon <span dir="ltr"><<a
                              moz-do-not-send="true"
                              href="mailto:vivien.delmon@creatis.insa-lyon.fr"
                              target="_blank">vivien.delmon@creatis.insa-lyon.fr</a>></span>
                          wrote:<br>
                          <blockquote class="gmail_quote"
                            style="margin:0 0 0 .8ex;border-left:1px
                            #ccc solid;padding-left:1ex">On 10/18/2013
                            12:08 AM, Bilal Tahir wrote:<br>
                            <blockquote class="gmail_quote"
                              style="margin:0 0 0 .8ex;border-left:1px
                              #ccc solid;padding-left:1ex"> Dear VV
                              community,<br>
                              <br>
                              ...
                              <div><br>
                                <br>
                                Also I would like to know if the LOLA11
                                paper below is the correct<br>
                                reference if I decide to use your method
                                for publications?<br>
                                <br>
                                <a moz-do-not-send="true"
href="http://lola11.com/Results/displayApproved?result=20110708160218_86_CREATIS_CLB_Lyon_graphcut&file=20110708160218_86_CREATIS_CLB_Lyon_graphcut_lola11.pdf"
                                  target="_blank">http://lola11.com/Results/displayApproved?result=20110708160218_86_CREATIS_CLB_Lyon_graphcut&file=20110708160218_86_CREATIS_CLB_Lyon_graphcut_lola11.pdf</a><br>
                              </div>
                            </blockquote>
                            <br>
                            Part of the work presented in this paper
                            have been implemented in clitkExtractLung
                            but you need to explicitly ask the tool to
                            use it :<br>
                            <br>
                            Step 2 : find trachea:<br>
                              --type=INT trachea finding algorithm (0:
                            original; 1:LOLA11) (default=`0')<br>
                            <br>
                            The second part about graphcut labeling were
                            not included in this tool since we never
                            really need to separate lungs.<br>
                            <br>
                            Last time I tried to activate --type=1 it
                            failed, I will have a look at it one day.<br>
                            <br>
                            As far as I know there is no other paper
                            that describe what we have implemented in
                            this tool, so this paper should be the one
                            to cite.<span><font color="#888888"><br>
                                <br>
                                -- <br>
                                Vivien Delmon<br>
                                PhD student at CREATIS, Lyon<br>
                                Supported by ANRT and ELEKTA<br>
                                <a moz-do-not-send="true"
                                  href="http://www.creatis.insa-lyon.fr/rio"
                                  target="_blank">http://www.creatis.insa-lyon.fr/rio</a></font></span>
                            <div>
                              <div><br>
_______________________________________________<br>
                                vv mailing list<br>
                                <a moz-do-not-send="true"
                                  href="mailto:vv@creatis.insa-lyon.fr"
                                  target="_blank">vv@creatis.insa-lyon.fr</a><br>
                                <a moz-do-not-send="true"
                                  href="http://www.creatis.insa-lyon.fr/mailman/listinfo/vv"
                                  target="_blank">http://www.creatis.insa-lyon.fr/mailman/listinfo/vv</a><br>
                              </div>
                            </div>
                          </blockquote>
                        </div>
                        <br>
                        <br clear="all">
                        <div><br>
                        </div>
                        -- <br>
                        <div dir="ltr">Bilal Tahir,
                          <div>James Morrison Researcher in Radiotherapy
                            Imaging,</div>
                          <div>Department of Clinical Oncology,</div>
                          <div>University of Sheffield</div>
                        </div>
                      </div>
                    </div>
                    <br>
                    <fieldset></fieldset>
                    <br>
                    <pre>_______________________________________________
vv mailing list
<a moz-do-not-send="true" href="mailto:vv@creatis.insa-lyon.fr" target="_blank">vv@creatis.insa-lyon.fr</a>
<a moz-do-not-send="true" href="http://www.creatis.insa-lyon.fr/mailman/listinfo/vv" target="_blank">http://www.creatis.insa-lyon.fr/mailman/listinfo/vv</a>
</pre>
                  </blockquote>
                  <br>
                </div>
              </div>
            </div>
          </blockquote>
        </div>
        <br>
        <br clear="all">
        <div><br>
        </div>
        -- <br>
        <div dir="ltr">Bilal Tahir,
          <div>James Morrison Researcher in Radiotherapy Imaging,</div>
          <div>Department of Clinical Oncology,</div>
          <div>University of Sheffield</div>
        </div>
      </div>
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